Oligonucleotide directed mutagenesis in biology
2:30
humix.com
Oligonucleotide directed mutagenesis in biology
Today, we delve into the world of oligonucleotide directed mutagenesis, a technique that revolutionized molecular biology. By precisely altering DNA sequences, scientists can unravel the mysteries of genes and their functions.
5 months ago
Site-directed mutagenesis Principles
Brianna Bibel on Instagram: "Q5 SDM: Site-Directed Mutagenesis using the NEB Q5 SDM Kit https://youtu.be/tqONxVkMHIs 1. Perform PCR with 1) a forward primer that incorporates the mutation you want and 2) a reverse primer that abuts the 5' end of the forward primer 2. Add the KLD mixture containing: 1. K: a Kinase to phosphorylate the 5' ends to allow for ligation (since the primers come unphosphorylated) 2. L: A Ligase to stitch the ends together, circularizing the PCR product 3. D: Donl to chew
13:10
Brianna Bibel on Instagram: "Q5 SDM: Site-Directed Mutagenesis using the NEB Q5 SDM Kit https://youtu.be/tqONxVkMHIs 1. Perform PCR with 1) a forward primer that incorporates the mutation you want and 2) a reverse primer that abuts the 5' end of the forward primer 2. Add the KLD mixture containing: 1. K: a Kinase to phosphorylate the 5' ends to allow for ligation (since the primers come unphosphorylated) 2. L: A Ligase to stitch the ends together, circularizing the PCR product 3. D: Donl to chew
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